atomic force microscope ir s-snom setup (modified afm)  (Bruker Corporation)

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Overview of experimental design ( a ) Schematic of P. margaritifera shell structure and composition at the interface between the nacre and the prismatic region. ( b ) Optical image of the nacre-prisms interfacial region indicated by the box in a). ( c ) A summary of select existing technologies applied to biomineralization imaging arranged by spatial resolution and specificity. ( d ) Experimental concept. Combined IR s -SNOM and NanoSIMS gives correlated chemical and elemental information with nanoscale spatial resolution.

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques: Imaging

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Spatio-spectral analysis of interlamellar organic layers in nacre ( a ) AFM height and ( b ) IR s- SNOM image at 1660 cm -1 mapping the amide I band in the nacre region. The prism-covering organic membrane extends ~ 30 μm into the nacre from the nacre/prismatic boundary. Near the end of the organic membrane, organic layers of ~ 50 nm in width appear with ~ 600 nm periodicity. ( c ) AFM topography and ( d ) IR s- SNOM of the subregion shown in ( a ) (black square). ( e ) Spatio-spectral transect along the dashed line in ( d ) demonstrating the amide I band with its spatial < 100 nm confinement in the interlamellar organic layer.

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques: Membrane

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Organic mapping and minerology of nacre region. ( a ) AFM topography of boundary regions between the prismatic and the nacre regions. Two ~ 10 μm grains appear at the aragonite-calcite boundary. Inset: IR s- SNOM point spectra acquired at the locations indicated (colored circles). The nacre region (black circle) and the crystallites (red circle) are identified as aragonite by their characteristic peak at 858 cm −1 , while the blue-shifted resonance at 876 cm −1 for the prismatic region (blue circle) is characteristic for calcite. ( b ) Corresponding amide I resonant IR s -SNOM image at 1660 cm −1 mapping the protein layers across this region. There are strong signatures near the boundary and extending outwards. Fainter striations begin near the end of the inclusion and extend into the nacre. The protein bands are periodic with ~ 600 nm spacing, as exemplified by the 2D FFT of the region shown in the inset.

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques:

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Minerology across the NPI. ( a ) Topography across the NPI, separated by the prism-covering membrane. s -SNOM images at ( b ) 860 cm –1 and ( c ) 880 cm −1 with contrast due to the distinct aragonite vs. calcite vibrational signatures. ( d ) Spatio-spectral linescan at the edge of the nacre shows the carbonite stretch at 860 cm −1 , as well as an organic signature at 930 cm −1 . ( e ) Spatio-spectral linecut along the prism edge with peak at 880 cm −1 indicative of calcite.

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques: Membrane

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Region with organic pattern and nacre tablet morphology. ( a ) AFM topography and ( b ) corresponding IR s -SNOM image acquired at 1660 cm −1 mapping the protein sheets across a ~ 60 μm region showing long range order. The regular periodic striations appear ~ 15 μm from the NPI to the left and right of the prism-covering membrane inclusions. However, the nacre tablets extend down to within ~ 5 μm of the NPI boundary in the center of the image, with decreasing regularity and contrast due to increased dispersed organics in the carbonate (red lines).

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques: Membrane

Journal: Scientific Reports

Article Title: Correlative chemical and elemental nano-imaging of morphology and disorder at the nacre-prismatic region interface in Pinctada margaritifera

doi: 10.1038/s41598-023-47446-5

Figure Lengend Snippet: Correlated IR s -SNOM, and NanoSIMS of nacre region. ( a ) The AFM height and ( b ) s -SNOM phase map at 1660 cm −1 of the terminus of a prism-covering matrix extension. Correlated NanoSIMS correlates the presence of ( c ) 12 C 14 N and ( d ) 40 Ca 16 O. ( j ) A correlated linecut of the s -SNOM and NanoSIMS images (white dashed line in ( c )) is displayed in ( e ) and shows the correlation between the amide-I response and 12 C 14 N, 12 C 12 C, and 12 C.

Article Snippet: The light passes through an interferometer and is focused onto the metallic tip (160AC-GG, Opus) of an atomic force microscope (AFM) based IR s -SNOM setup (modified AFM+, Bruker).

Techniques: